Regular CIP also prevents the build-up of these impurities in the chromatography bed and helps maintain the capacity, flow properties, and general performance of the medium. Scalable results to BioProcess columns using the same linear flow velocityĬleaning in place (CIP) is a cleaning procedure to remove impurities such as lipids, precipitates, or denatured proteins that can remain in the packed column after regeneration.Easy connection of two columns in series for 20 cm bed height.Excellent for method optimization due to the 10 cm bed height.Sold as 5 × 1 mL, 2 × 1 mL, 1 × 5 mL and 5 × 5 mL depending on the medium.To be used manually (syringe), with a peristaltic pump, or a system such as ÄKTA. Broad range of prepacked chromatography media.Suitable for condition screening and small-scale purifications. Sold as eight columns in row per package.Column format: Prepacked as 200 μL or 600 μL.PreDictor RoboColumn miniaturized columns To be used with a robot or manually (centrifugation).Excellent for high-throughput chromatography media screening.Excellent for high-throughput condition/parameter screening.Format: Prefilled 96-well filter plate, several different media volumes/well depending on the specific media.The small format volumes give fast results and low sample/buffer consumption. Protein A at 90% monomer recovery (ppm) (start ~ 1 ppm)Įlution pool concentration at 90% monomer recovery (mg/mL)Īll are prepacked, convenient, ready-to-use formats for fast and reproducible process development and small-scale purifications. HCP at 90% monomer recovery (ppm) (start 1800 ppm) Summary of results for chromatogram shown in Figure 8. Blue: absorbance at 280 nm, red: MAb aggregates, green: HCP, orange: conductivity. Chromatogram from purification of a MAb on Capto S ImpAct, demonstrating the high resolution even at high load. Linear, 0 to 350 mM NaCl (0% to 70% elution buffer) in 20 CVįig 8. pH adjusted to pH 5.3ĥ0 mM sodium acetate, 500 mM NaCl, pH 5.3 Running conditionsĩ.6 mg/mL MAb C, purified on MabSelect SuRe medium. The chromatogram shows the analysis of the aggregate (in red) and host cell protein (HCP) contents (in green). See also data file 29-0670-18, and application note “Polishing of monoclonal antibodies using Capto S ImpAct”, 29-1083-27.įigure 8 shows the resolution for Capto S ImpAct at 70% of DBC at 10% breakthrough (64 mg/mL for Capto S ImpAct). Green lines show the residence time in the column (4, 8, and 16 min). Data corresponds to a production-scale column at 20☌ and a viscosity equivalent to water. The pressure/flow window of operation (area under the curve) of Capto S ImpAct. For recommended columns, see data file, 29-0670-18.įig 6. Note that the maximum operating velocity of Capto S ImpAct is dependent of the column used as well as the viscosity of the liquid. Capto S ImpAct chromatography medium can normally be run at maximum pressure ratings of low-pressure columns (3 bar ). The pressure limits for Capto S ImpAct shown in Figure 6 are based on a production-scale column and are calculated for 20 cm bed height and maximum operating flow velocities of 220 cm/h.Īt this flow velocity, the pressure is equal to or less than 3 bar (0.3 MPa) measured using process buffers with the same viscosity as water, 20☌, which is the highest recommended operating pressure for this medium at this scale. The size of the area below the pressure-limit curve represents the window of operation, that is, the available operating range for the medium. Higher bed heights mean smaller diameters and a reduced column footprint.įigure 6 shows the relationship between column bed height and operating flow velocity for Capto S ImpAct. High flow velocities increase volume throughput and decrease process time. Properties of Capto media, such as matrix rigidity, allow a wide working range of flow velocities, bed heights, and sample viscosities.
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